I wish I could give you measurements...I know engineers need measurements (my Dad is an engineer- missiles and medical devices for over 40 years).
However, my bike is completely dissasemmbled to be painted. I can only talk in generalities. My frame is a standard medium cruiser frame of the cantilever variety (it's under there somewhere), and I had absolutely no problem clearing the seat tube. There was probably a little over 25" clearance. Doesn't sound like much, but it's plenty.
What created some clearance issues for me was the tire (as mentioned above), but I am using 26 x 2.125. Even then, I was able to set it up to clear the tire by over 1/8" (ok, engineer... .125"). This required me to move the engine as far to the left as possible. All the way to the left in the slots, and then I tightened the right side plate to block bolts first so it pushes the plate another 3/16" to the left. Then I tightened the left side.
The alignment for the drive sprocket to the wheel sprocket is fine. I use a #41 chain that provides a little more space between the plates and I ran this setup for enough miles mocked up (probably 100) to know that the chain stays where it is supposed to.
I must use the chain tensioner, however, to gide the chain a bit so as to not rub on the wheelstay.
If I can find any pics that show the areas at issue, I will post them.