Should always mix your fuel and oil in a gallon container, shaken vigorously so it's completely mixed in.
I don't recommend mixing it in the bike's fuel tank as invariably the oil will seek the lowest part of the tank and concentrate some the fuel oil mix in the fuel line to carburetor, before the fuel gets to mix completely with the rest of the oil in the tank. Since the fuel lines are pretty big in diameter, you could possibly loose almost an ounce of oil to the fuel line. That could change a 32:1 premix ratio to over 40:1.
Always mix it separately.